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听力学及言语疾病杂志JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2003 Vol.11 No.4 P.281-283
卡铂对离体培养成年灰鼠前庭毛细胞的损害
Carboplatin Induced Vestibular Hair Cell Lesion in the Adult Chinchilla Organotypic Cultures
丁大连
蒋海燕
王家东
杨军
Richard J.Salvi
摘 要：目的观察并建立不同浓度卡铂损害成年灰鼠前庭终器的离体实验模型.方法应用前庭终器分离取材技术、前庭器官离体培养技术和组织学检查技术,观察不同浓度卡铂对成年灰鼠前庭各终器的损害.结果卡铂主要损害灰鼠前庭Ⅰ型毛细胞,这种损害随着卡铂剂量的增加而加重.结论卡铂选择性破坏离体培养的灰鼠前庭Ⅰ型毛细胞.
关键词：器官培养 卡铂 前庭毛细胞
文章编号：1006-7299(2003)04-0281-03
基金项目：国家自然科学基金资助课题(编号 30100206)
作者简介：丁大连,男,辽宁人,研究员,主要从事耳毒性药物对耳蜗及前庭损害的形态听功能研究
作者单位：丁大连（Center for Hearing and Deafness,State University of New York at Buffalo）
蒋海燕（Center for Hearing and Deafness,State University of New York at Buffalo）
王家东（上海第二医科大学附属仁济医院耳鼻咽喉-头颈外科）
杨军（上海第二医科大学附属新华医院耳鼻咽喉-头颈外科）
Richard J.Salvi（Center for Hearing and Deafness,State University of New York at Buffalo）
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