请后下载此文档！
听力学及言语疾病杂志JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2003 Vol.11 No.4 P.286-287,294
庆大霉素对豚鼠耳蜗传入神经损伤的电镜观察
Ultrastructural Evaluation of Gentamicin Toxicity on Cochlear Afferent Never System of Guinea Pig
邓志宏
刘顺利
王锦玲
邱建华
黄晓峰
黄梅
摘 要：目的观察庆大霉素损伤对豚鼠耳蜗传入神经超微结构的影响.方法听力正常的豚鼠肌注硫酸庆大霉素(120mg/kg),连续注射14天,分别于停药后1天、2周在透射电镜下观察传入神经超微结构改变,并进行ABR测听.结果停药1天时,ABR阈值与正常对照组相比,阈值明显升高(P＜0.05),停药2周时,ABR阈值进一步升高,与1天时相比,亦有显著性差异(P＜0.05).庆大霉素损伤后,随着毛细胞的损伤,传入神经末梢肿胀、突触间隙增大,传入神经纤维及螺旋神经节损伤程度依次减轻;随着损伤时间的延长,传入神经末梢的病变加重,相应的神经纤维及螺旋神经节的损伤亦加重.结论在庆大霉素作用下,随着毛细胞损伤的加重,耳蜗传入神经损伤加重,并出现不可逆转性病理改变,与阈值升高有关.
关键词：庆大霉素 耳蜗 传入神经 电镜
文章编号：1006-7299(2003)04-0286-03
作者简介：邓志宏,女,辽宁人,医学博士,主要从事耳鼻咽喉科临床和听力学基础研究
作者单位：邓志宏（第四军医大学西京医院耳鼻咽喉科,西安,710032）
刘顺利（第四军医大学西京医院耳鼻咽喉科,西安,710032）
王锦玲（第四军医大学西京医院耳鼻咽喉科,西安,710032）
邱建华（第四军医大学西京医院耳鼻咽喉科,西安,710032）
黄晓峰（第四军医大学电镜室）
黄梅（第四军医大学电镜室）
参考文献：
［1］Sun H, Hashino E, Ding DL, et al. Reversible and irreversible damage to cochlear afferent netrons by kainic acid excitotoxicity [ J ]. J Comp Neurol,2001,430:172.
［2］Cazals Y. Auditory sensori - neural alterations induced by salicylate[J] .Prog Neurobiol,2000,62:583.
［3］d'Aldin CG, Ruel J, AssieR,etal. Implication of NMDA type glutamate receptors in neural regeneration and neoformation of synapses after excitotoxic injury in the guinea pig cochlea[J]. Int J Dev Neurosci,1997,15:619.
［4］Felix H. Anatomical differences in the peripheral auditory system of mammals and man[ J]. Adv Otorhinolaryngol,2002,59:1.
［5］Slepecky NB, Galsky MD, Swartzentruber- Martin H, et al. Study of afferent nerve terminals and fibers in the gerbil cochlea: distribution by size[J]. Hear Res,2000,144:124.
［6］丁大连,李明,姜泗长,等.内耳形态学[M].哈尔滨:黑龙江科学技术出版社,2001,55-66.
［7］Gaborjan A, Lendvai B, Vizi ES. Neuroehemical evidence of dopamine release by lateral olivocochlear efferents and its presynaptic modulafion in guinea - pig cochlea[J]. Neuroscience, 1999,90:131.
［8］Nordmann AS, Bohne BA, Handing GW. Histopathological differences between temporary and permanent threshold shift[ J]. Hear Res,2000,139:13.
［9］Johnson KL, CarrascoV, PrazmaJ,etal. Role of nitric oxide in kainic acid - induced elevation of cochlear compound action potential thresholds[J]. Acta Otolaryngol, 1998,118:660.
［10］王大君,韩东一,杨伟炎,等.硫酸庆大霉毒对小鼠耳蜗螺旋神经节细胞生长的影响[J].临床耳鼻咽喉科杂志,1998,12:320.
［11］Shinohara T, Bredberg G, Ulfendahl M,et al. Neurotrophic factor intervention restores auditory function in deafened animals [ J ]. Proc Natl Acad Sci U S A , 2002,99:1 657.