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听力学及言语疾病杂志JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2002 Vol.10 No.3 P.158-160
组织块培养法及胶原酶消化法分离培养豚鼠耳蜗微血管内皮细胞
Isolation and Cultivation of Cochlear Microvascular Endothelial Cells from Guinea Pigs by Tissue Nubbles Cultivation and Collagenase Digestion.
魏运军
张学渊
摘 要：目的建立稳定的豚鼠耳蜗微血管内皮细胞的培养方法.方法采用显微解剖技术分离出豚鼠耳蜗血管纹,分别用组织块法及酶消化法进行培养及纯化.结果耳蜗血管纹组织块培养后2天,部分组织块边缘有散在的细胞生长,这些细胞逐渐增殖形成大片细胞集落;耳蜗血管纹组织经胶原酶消化后1～3天,可观察到培养皿底由一些细胞组成的细胞岛;细胞纯化后大多呈多边形,致密融合时具有内皮细胞培养时典型的"铺路石样"外观,经免疫组化检测其内皮细胞标志性抗原Ⅷ因子,95%以上的培养细胞的胞浆中呈棕黄色阳性反应.结论组织块法及酶消化法能够获得体外培养的豚鼠耳蜗微血管内皮细胞.
关键词：豚鼠 血管纹 内皮细胞 细胞培养 免疫细胞化学染色
文章编号：1006-7299(2002)03-0158-03
基金项目：国家自然科学基金资助项目(项目编号 39870834)
作者简介：魏运军(1965-),男,湖北人,讲师,主治医师,主要从事内耳微循环方面的研究
作者单位：魏运军（第三军医大学附属西南医院耳鼻咽喉科,重庆,400038）
张学渊（第三军医大学附属西南医院耳鼻咽喉科,重庆,400038）
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