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听力学及言语疾病杂志JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
2002 Vol.10 No.4 P.241-243
慢性庆大霉素损伤后豚鼠前庭上皮细胞的凋亡和再生观察
Apoptosis and Proliferation Following Chronic Gentamicin Toxicity in Guinea Pig Vestibular Sensory Epithelium
马晓莉
黄维国
王锦玲
姜鸿彦
刘顺利
邱建华
摘 要：目的观察慢性庆大霉素中毒后豚鼠前庭上皮的损伤和修复过程,探讨前庭毛细胞凋亡和再生的关系.方法采用末端脱氧核苷酸转移酶介导的 dUTP 缺口末端标记(terminal deoxyuncleotidyl transferase-mediate d deoxyuridine triphosphate nick end labeling, TUNEL)法,观察前庭感觉上皮细胞的 凋亡,采用5'-溴-2-脱氧尿苷(bromodeoxyuridine, Brdu)免疫细胞化学法观察前庭 上皮的再生过程.结果对照组没有观察到TUNEL和Brdu阳性细胞 .停止庆大霉素注射1天至3周均可观察到 TUNEL 阳性细胞,但1天组阳性细胞数量最多,3 周仍有阳性细胞存在,阳性细胞主要位于毛细胞层.停止庆大霉素注射后1天组支持细胞层和毛细胞层均有 Brdu 标记阳性细胞,3 d 组支持细胞层阳性细胞显著增多,1周组毛细胞层也出现较多阳性细胞,2周组 Brdu 标记阳性细胞主要位于毛细胞层,3周仍可观察到 Brdu 标记阳性细胞.结论慢性庆大霉素耳中毒后豚鼠前庭上皮细胞凋 亡增加,凋亡与增殖伴随存在,前庭毛细胞的损伤可能是其增殖的触发因素.增殖过程表现 为支持细胞增生向毛细胞增生移行的趋势,支持细胞增殖并转化成毛细胞可能是前庭上皮细胞损伤修复机理之一.
关键词：前庭感觉上皮 凋亡 再生 庆大霉素
文章编号：1006-7299(2002)04-0241-03
基金项目：国家自然科学基金资助项目(39870788)
作者简介：马晓莉(1972-),女,黑龙江人,医学硕士,主要从事耳鼻咽喉科临床与听力学研究
作者单位：马晓莉（空军总医院耳鼻咽喉科,北京,100036）
黄维国（第四军医大学西京医院耳鼻咽喉科）
王锦玲（第四军医大学西京医院耳鼻咽喉科;空军总医院耳鼻咽喉科,北京,100036）
姜鸿彦（第四军医大学西京医院耳鼻咽喉科）
刘顺利（第四军医大学西京医院耳鼻咽喉科）
邱建华（第四军医大学西京医院耳鼻咽喉科）
参考文献：
［1］Nakagawa T, Yamane H, Takayama M. Time-dependent response of ves tibular hair cells of guinea pigs following high-dose applications of sterptom ycin[J]. Acta Otolaryngol Suppl Stockh, 1998, 538:32.
［2］Zheng JL, Keller G, Gao WQ. Immunocytochemical and morphological evi dence for intracellular self-repair as an important contributor to mammalian h air cell recovery[J]. J Neurosci, 1999, 19:2161.
［3］Lang H, Liu C, Fan E. Bromodeoxyuridine immunohistochemical observat ion of sensory epithelium rapair after ototoxic destruction in the crista ampull aris of guinea pigs[J]. Chin J Otorhinolaryngol, 1996, 31:341.
［4］Lang H, Liu C. Apoptosis and hair cell degeneration in the vestibula r sensory epithelia of the guinea pig following a gentamicin insult[J]. Hear R es, 1997, 111:177.
［5］Forge A, Li L. Apoptotic death of hair cells in mammalian vestibular sensory epithelia[J]. Hear - Res, 2000, 139:97.
［6］Lopez I, Honrubia V, Lee SC. Quantification of the process of hair c ell olss and recovery in the chinchilla crista ampullaris after gentamicin tretm ent[J]. Int J Dev Neurosci, 1997, 15:447.
［7］Yamane H, Nakagawa T, Iguchi H. In vivo regeneration of vestibular h air cells of guinea pig[J]. Acta Otolaryngol Suppl Stockh,1995, 520:174.
［8］Li L, Forge A. Morphological evidence for supporting cell to hair ce ll conversion in the mammalian utricular macula[J]. Int J Dev Neurosci, 1997, 15:433.
［9］Forge A, Li L, Nevill G. Hair cell recovery in the vestibular sensor y epithelia of mature guinea pigs[J]. J Comp Neurol, 1998, 397:69.
［10］Gray EMG, Warchol ME, Corwin JF. IGF - 1 protects hair cell from a m inoglycoside - induced apoptotic cell death[J]. Assoc Res Otolaryngol Abstr, 1 996, 19:198.
［11］Li L, Forge A, Nevill G. Apoptotic death of hair cells[J]. Assoc Res Otolaryngol Abstr, 1997, 20:112.
［12］Lambert PR. Inner ear hair cell regeneration in a mammal: identific ation of a triggering factor[J]. Laryngoscope, 1994, 104:701.
［13］Yamashita H, Oesterle EC. Induction of cell proliferation in mammali an inner - ear sensory epithelia by transforming growth factor alpha and epidema l growth factor[J]. Proc Natl Acad Sci U S A, 1995, 92:3152.
［14］Hockenbery D, Nunes G, Milliman C. Bcl - 2 is an inner mitochondria l membrane protein that blocks programmed cell death[J]. Nature, 1990, 348:334 .
［15］Garcia I, Martinou I, Tsulimono Y. Prevention of programmed cell de ath of sympathetic neurons by the bcl-2 protonncogene[J]. Science, 1992, 258: 302.
［16］Fekete DM, Homburger S, Waring MT. Involvement of programmed cell d eath in morphogenesis of the vertebrate inner ear[J]. Development, 1997, 124:2451.